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Gastroparesis and lipid metabolism-associated dysbiosis in Wistar-Kyoto rats

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posted on 2023-05-03, 10:53 authored by Julie DalzielJulie Dalziel, Karl FraserKarl Fraser, Wayne Young, Catherine McKenzie, Shalome Bassett, Nicole Roy
Altered gastric accommodation and intestinal morphology suggests impaired gastrointestinal (GI) transit may occur in the Wistar Kyoto (WKY) rat strain, as common in stress-associated functional GI disorders. Because changes in GI transit can alter microbiota composition, we investigated whether these are altered in WKY rats compared with the resilient Sprague Dawley (SD) rats under basal conditions, and characterized plasma lipid and metabolite differences. Bead transit was tracked by X-ray imaging to monitor: gastric emptying (GE; 4 h), small intestine (SI) transit (9 h) and large intestine transit (12 h). Plasma extracts were analysed by lipid and HILIC LC-MS. Cecal microbial composition was determined by Illumina MiSeq 16S rRNA amplicon sequencing and analysis using the QIIME pipeline. Stomach retention of beads was 77% for WKY compared with 35% for SD rats. GI transit was decreased by 34% (9 h), and 21% (12 h) in WKY compared with SD rats. Excluding stomach retention, transiting beads moved 29% further along the SI over 4-9 h for WKY compared with SD rats. Cecal Ruminococcus, Roseburia, and unclassified Lachnospiraceae genera were less abundant in WKY rats, whereas the minor taxa Dorea, Turicibacter, and Lactobacillus were higher. Diglycerides, triglycerides, phosphatidyl-ethanolamines and phosphatidylserine were lower in WKY rats, whereas cholesterol esters and taurocholic acids were higher. The unexpected WKY rat phenotype of delayed gastric emptying, yet rapid SI transit, was associated with altered lipid and metabolite profiles. The delayed gastric emptying of the WKY phenotype suggests this rat strain may be useful as a model for gastroparesis.

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Rights statement

Copyright © 2017 the American Physiological Society. Licensed under Creative Commons Attribution CC-BY 4.0: © the American Physiological Society.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

American Physiological Society

Journal title

American Journal of Physiology. Gastrointestinal and liver physiology

ISSN

0193-1857

Citation

Dalziel, J. E., Fraser, K., Young, W., McKenzie, C. M., Bassett, S. A., & Roy, N. C. (2017). Gastroparesis and lipid metabolism-associated dysbiosis in Wistar-Kyoto rats. American Journal of Physiology. Gastrointestinal and Liver Physiology, 313(1), G62–G72. doi:10.1152/ajpgi.00008.2017

Funder

Core Funding

Contract number

A21246

Job code

11574

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