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Efferent intestinal lymph protein responses in nematode-resistant, -resilient and -susceptible lambs under challenge with Trichostrongylus colubriformis

journal contribution
posted on 2023-05-03, 11:40 authored by Jude Bond, Tony Pernthaner, K Zhang, Sarah RosanowskiSarah Rosanowski, Stefan ClerensStefan Clerens, Stewart Bisset, Ian SutherlandIan Sutherland, John Koolaard, Wayne Hein
The mechanisms underlying resistance to challenge by gastrointestinal nematode parasites in sheep are complex. Using DIGE, we profiled ovine lymph proteins in lambs with host resistance (R), resilience (Ri) or susceptibility (S) to a daily trickle challenge with the nematode Trichostrongylus colubriformis. Efferent intestinal lymph was collected prior to infection (day 1) and on days 5 and 10 post-infection. Eight proteins identified by LC-MS/MS, showed differences relating to host genotype. Of these, Serpin A3-3 and Serpin A3-7 have not been reported previously in the lymph proteome. Three acute phase proteins showed significant differences relating to interactions between breeding line and parasite challenge, including complement C3β, C3α and haptoglobin (Hp) β. In the R lambs C3α was significantly up regulated (P < 0.05) on day 10, while in the Ri lambs Hp β was significantly down regulated (P < 0.05). In the S lambs, levels of C3β were up regulated and levels of Hp β down regulated (both P < 0.05) on day 10. Hence we demonstrate that acute phase inflammation proteins contribute to differences in the innate immune response of sheep to challenge by T. colubriformis. The findings may lead to the development of new approaches to combat nematode infestations in sheep production systems. Biological Significance Breeding lines of sheep with resistance (R), resilience (Ri) or susceptibility (S) to nematode infections provide an experimental model to examine the biological mechanisms underlying the ability of some sheep to expel worms and remain healthy without the use of an anthelmintic. Using proteomics we identified differences in the expression of acute phase lymph proteins in the R, Ri and S lambs. The results will assist the development of alternative control strategies to manage nematode infections in livestock.

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Rights statement

© 2014 Elsevier B.V. All rights reserved.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Elsevier

Journal title

Journal of Proteomics

ISSN

1874-3919

Citation

Bond, J. J., Pernthaner, A., Zhang, K., Rosanowski, S. M., Clerens, S., Bisset, S. A., Sutherland, I.A.S., Koolaard, J.P. & Hein, W. R. (2014). Efferent intestinal lymph protein responses in nematode-resistant, -resilient and -susceptible lambs under challenge with Trichostrongylus colubriformis. Journal of Proteomics, 109, 356-367.

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