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Marroni 2023 Real time PCR to detect ROD in Metrosideros.pdf (481.42 kB)

Validation of a real-time PCR protocol to detect Ceratocystis lukuohia and C. huliohia from Aotearoa New Zealand Metrosideros species

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posted on 2023-08-17, 01:48 authored by Virginia Marroni, Sandi Keenan, Eva Brill, Mark McNeillMark McNeill, Teresa Waiariki, Wai Awarau-MorrisWai Awarau-Morris, Lisa Keith

Ceratocystis lukuohia, the cause of ōhi’a wilt disease, and C. huliohia, the cause of ōhi’a canker disease (collectively known as rapid ōhi’a death or ROD), have resulted in widespread death of ‘ōhi’a lehua (Metrosideros polymorpha) in Hawai’i. Aotearoa New Zealand Metrosideros species, such as the iconic pōhutukawa and rātā, are closely related to M. polymorpha. The pathogens are not currently present in Aotearoa New Zealand but should either of these pathogens establish in this country, they could affect endemic and indigenous Metrosideros species. In this study, the use of a real-time PCR protocol, developed in Hawai’i to detect and differentiate C. lukuohia and C. huliohia from ōhi’a plant tissue, was validated for three Metrosideros species from Aotearoa New Zealand.

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Rights statement

This is an open-access output. It may be used, distributed or reproduced in any medium, provided the original author and source are credited.

Publication date

2023-05-01

Project number

  • Non revenue

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

AgResearch Ltd

Conference name

Fourth International Congress on Biological Invasions (ICBI 2023)

Conference location

Christchurch, New Zealand

Conference start date

2023-05-01

Conference end date

2023-05-05

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