Impact of cell wall polysaccharide modifications on the performance of <i>Pichia pastoris</i> GS115: novel mutants with enhanced bioproduction

<p dir="ltr"><b>Highlights</b></p><ol><li>Two novel <i>P. pastoris</i> chassis hosts with impaired β-1,3-D-glucan biosynthesis were developed (H001 and H002). Reducing β-glucan content in the cell wall of the two GS115 mutants affected cell wall structure.</li><li>Enhanced recombinant green fluorescent protein (GFP) production was detected in H001/H002 .</li><li>H001 and H002 show faster growth and higher carbon-to-biomass conversion.</li><li>Increased S-adenosyl-L-methionine (SAM) synthesis in H001/H002 was achieved.</li><li>Significant variations in lipid composition were observed between GS115 and the two modified hosts.</li></ol><p dir="ltr"><b>Aim</b>: To investigate how alterations in cell wall/membrane affect the fitness and function of <i>P. pastoris</i>, contributing to a better understanding of its overall capabilities.</p><p dir="ltr"><br></p><p dir="ltr"><br></p>