File(s) not publicly available

pH-induced interfacial properties of Chaplin E from Streptomyces coelicolor

journal contribution
posted on 2023-05-03, 10:03 authored by Mina Dokouhaki, Andrew Hung, Emma Prime, Greg Qiao, Li DayLi Day, Sally Gras
Chaplin E, or Chp E, is a surface active peptide secreted by Streptomyces coelicolor that adopts different structures depending on solution pH but the effect of these structures on the interfacial properties of Chp E is not known. In experiments paired with simulations, Chp E was found to display pH-dependent inter-facial assembly and surface activity. At pH 3.0, Chp E formed an ordered non-amyloidal interfacial film with high surface activity; while at pH 10.0, Chp E self-assembled into a heterogeneous film containing randomly arranged fibrils at the interface that was less surface active compared to the film formed at pH 3.0. In simulations at pH 10.0, Chp E molecules showed a higher propensity for dimerization within the solution phase, lower rate of adsorption to the interface and tighter inter-molecular associations at the interface, consistent with the lower surface activity and smaller interfacial area coverage per molecule measured at this pH compared to at pH 3.0. A model is presented for the role of Chp E in the develop-mental differentiation of Streptomyces coelicolor, where Chp E contributes to changes in surface tension at low pH and the formation of fibrils on the surface of aerial hyphae at high pH. Our data also suggest Chp E could be a promising surface active agent with functional activity that can be controlled by pH.


Rights statement

© 2017 Elsevier B.V. All rights reserved.


  • English

Does this contain Māori information or data?

  • No



Journal title

Colloids and Surfaces B: Biointerfaces




Dokouhaki, M., Hung, A., Prime, E. L., Qiao, G. G., Day, L., & Gras, S. L. (2017). pH-induced interfacial properties of Chaplin E from Streptomyces coelicolor. Colloids and Surfaces B: Biointerfaces, 160, 589–597. doi:10.1016/j.colsurfb.2017.10.006

Report number

FBP 78175

Usage metrics


    Ref. manager