4 files

Whole Genome Sequencing for Determining the Source of Mycobacterium bovis infections in livestock herds and wildlife in New Zealand

journal contribution
posted on 2023-05-03, 16:44 authored by Marian Price-CarterMarian Price-Carter, Rudiger BrauningRudiger Brauning, Geoff DeLisle, Paul Livingstone, Mark Neill, Jane Sinclair, Brent Paterson, Garry Knowles, Gillian Atkinson, Kevin Crews, Rowland Kao, Suelee Robbe-Austerman, Tod Stuber, Simon Haris, Julian Parkhill, James Wood, Des Collins
The ability to DNA fingerprint Mycobacterium bovis (M. bovis) isolates helped to define the role of wildlife in the spread of bovine tuberculosis in New Zealand. DNA fingerprinting results currently help to guide wildlife control measures and also aid in tracing the source of infections that result from movement of livestock. During the last 5 years we have developed the ability to distinguish New Zealand (NZ) M. bovis isolates by comparing the sequences of whole genome sequenced (WGS) M. bovis samples. WGS provides much higher resolution than our other established typing methods and greatly improved the definition of the regional localization of NZ M. bovis types. Three outbreak investigations are described and results demonstrate how WGS analysis has led to the confirmation of epidemiological sourcing of infection, to better definition of the source of new infections by ruling out other possible sources, and has revealed probable wildlife infection in an area considered to be free of infected wildlife. The routine use of WGS analyses for sourcing new M. bovis infections will be an important component of the strategy employed to eradicate bovine TB from NZ livestock and wildlife.


Rights statement

Copyright © 2018 Price-Carter, Brauning, de Lisle, Livingstone, Neill, Sinclair, Paterson, Atkinson, Knowles, Crews, Crispell, Kao, Robbe-Austerman, Stuber, Parkhill, Wood, Harris and Collins. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.


  • English

Does this contain Māori information or data?

  • No


Frontiers Media

Journal title

Frontiers in Veterinary Science




Price-Carter, M., Brauning, R., DeLisle, G., Livingstone, P., Neill, M., Sinclair, J., … Collins, D. (2018). Whole Genome Sequencing for Determining the Source of Mycobacterium bovis infections in livestock herds and wildlife in New Zealand. Frontiers in Veterinary Science, 5, 272. doi:10.3389/fvets.2018.00272

Contract number


Job code


Usage metrics


    Ref. manager