Jones CVI 2017.pdf (1.05 MB)

Tuberculin skin testing boosts interferon gamma responses to DIVA reagents in Mycobacterium bovis-infected cattle.

Download (1.05 MB)
journal contribution
posted on 2023-05-03, 12:29 authored by Gareth Jones, Mick Coad, Bhagwati Khatri, Javier Bezos, Natalie ParlaneNatalie Parlane, Bryce BuddleBryce Buddle, Bernardo Villarreal-Ramos, R. Glynn Hewinson, H. Martin Vordermeier
Mycobacterium bovis BCG vaccination sensitizes cattle to bovine tuberculin, which compromises the use of the current bovine tuberculosis (TB) surveillance tests. Although the performance of a blood test (that utilizes antigens expressed by Mycobacterium bovis but not by BCG) capable of discriminating infected from vaccinated animals (DIVA interferon gamma test [DIT]) has been evaluated in naturally infected TB field reactors, there is a need to perform similar analysis in a BCG-vaccinated M. bovis-infected population. Furthermore, we explored different scenarios under which a DIT may be implemented alongside BCG vaccination: (i) serial testing to resolve potential false-positive skin test results or (ii) a standalone test to replace the single intradermal comparative cervical tuberculin (SICCT) skin test. Our results demonstrated significantly better relative test sensitivity when the DIT was evaluated in a serial test scenario. Direct comparison of pre-and post-skin test blood samples revealed that the SICCT test induced significant boosting of the gamma interferon response in M. bovis-infected animals to both the ESAT-6-CFP-10 and Rv3615c peptide cocktails that comprise the DIT, which persisted for the ESAT-6-CFP-10 reagent for at least 14 days. Importantly, no similar boosting effects were observed in noninfected BCG vaccinates, suggesting that DIVA blood testing after a recent skin test would have minimal impact on test specificity.


Rights statement

© Crown copyright 2017. The government of Australia, Canada, or the UK (“the Crown”) owns the copyright interests of authors who are government employees.


  • English

Does this contain Māori information or data?

  • No


American Society for Microbiology

Journal title

Clinical and Vaccine Immunology




Jones, G. J., Coad, M., Khatri, B., Bezos, J., Parlane, N. A., Buddle, B. M., Villarreal-Ramos, B., Hewinson, R. G., & Vordermeier, H. M. (2017). Tuberculin skin testing boosts interferon gamma responses to DIVA reagents in Mycobacterium bovis-infected cattle. Clinical and Vaccine Immunology, 24(5), e00551–16. doi:10.1128/CVI.00551-16


TBfree New Zealand

Contract number


Job code


Usage metrics


    Ref. manager