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Transcriptome changes of Escherichia coli, Enterococcus faecalis, and Escherichia coli O157:H7 laboratory strains in response to photo-degrated DOM

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posted on 2023-05-03, 12:06 authored by Adelumola Oladeinde, Erin Lipp, Chai-Ying Chen, Richard MuirheadRichard Muirhead, Travis Glenn, Kimberly Cook, Marirosa Molina
In this study, we investigated gene expression changes in three bacterial strains (Escherichia coli C3000, Escherichia coli O157:H7 B6914, and Enterococcus faecalis ATCC 29212), commonly used as indicators of water quality and as control strains in clinical, food, and water microbiology laboratories. Bacterial transcriptome responses from pure cultures were monitored in microcosms containing water amended with manure-derived dissolved organic matter (DOM), previously exposed to simulated sunlight for 12 h. We used RNA sequencing (RNA-seq) and quantitative real-time reverse transcriptase (qRT-PCR) to compare differentially expressed temporal transcripts between bacteria incubated in microcosms containing sunlight irradiated and non-irradiated DOM, for up to 24 h. In addition, we used whole genome sequencing simultaneously with RNA-seq to identify single nucleotide variants (SNV) acquired in bacterial populations during incubation. These results indicate that E. coli and E. faecalis have different mechanisms for removal of reactive oxygen species (ROS) produced from irradiated DOM. They are also able to produce micromolar concentrations of H2O2 from non-irradiated DOM, that should be detrimental to other bacteria present in the environment. Notably, this study provides an assessment of the role of two conjugative plasmids carried by the E. faecalis and highlights the differences in the overall survival dynamics of environmentally-relevant bacteria in the presence of naturally-produced ROS.


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© 2018 Oladeinde, Lipp, Chen, Muirhead, Glenn, Cook and Molina. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.


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Frontiers Media

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Frontiers in Microbiology




Oladeinde, A., Lipp, E., Chen, C.-Y., Muirhead, R., Glenn, T., Cook, K., & Molina, M. (2018). Transcriptome changes of Escherichia coli, Enterococcus faecalis, and Escherichia coli O157:H7 laboratory strains in response to photo-degrated DOM. Frontiers in Microbiology, 9, 882. doi:10.3389/fmicb.2018.00882


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