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Transcobalamin derived from bovine milk stimulates apical uptake of vitamin B12 into human intestinal epithelial cells.

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posted on 2023-05-03, 10:59 authored by Brad Hine, Irina Boggs, Ralph Green, Joshua Miller, Russell Hovey
Intestinal uptake of vitamin B12 (hereafter B12) is impaired in a significant proportion of the human population. This impairment is due to inherited or acquired defects in the expression or function of proteins involved in the binding of diet-derived B12 and its uptake into intestinal cells. Bovine milk is an abundant source of bioavailable B12 wherein it is complexed with transcobalamin. In humans, transcobalamin functions primarily as a circulatory protein, which binds B12 following its absorption and delivers it to peripheral tissues via its cognate receptor, CD320. In the current study, the transcobalamin-B12 complex was purified from cows' milk and its ability to stimulate uptake of B12 into cultured bovine, mouse and human cell lines was assessed. Bovine milk-derived transcobalamin-B12 complex was absorbed by all cell types tested, suggesting that the uptake mechanism is conserved across species. Furthermore, the complex stimulated the uptake of B12 via the apical surface of differentiated Caco-2 human intestinal epithelial cells. These findings suggest the presence of an alternative transcobalamin-mediated uptake pathway for B12 in the human intestine other than that mediated by the gastric glycoprotein, intrinsic factor. Our findings highlight the potential for transcobalamin-B12 complex derived from bovine milk to be used as a natural bioavailable alternative to orally administered free B12 to overcome B12 malabsorption

History

Rights statement

© 2014 Wiley Periodicals, Inc.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Wiley

Journal title

Cellular Biochemistry

ISSN

0730-2312

Citation

Hine, B., Boggs, I., Green, R., Miller, J.W., Hovey, R.C., Humphrey, R., & Wheeler, T. T. (2014), Transcobalamin derived from bovine milk stimulates apical uptake of vitamin B12 into human intestinal epithelial cells. Journal of Cellular Biochemistry, 115, 1948-1954. doi: 10.1002/jcb.24866

Funder

Ministry of Business Innovation & Employment;

Contract number

A12396

Job code

13371

Report number

FBP 45113

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