the-probiotic-lacticaseibacillus-rhamnosus-hn001-influences-the-architecture-and-gene-expression-of-small-intestine-tissue-in-a-piglet-model.pdf (420 kB)

The probiotic Lacticaseibacillus rhamnosus HN001 influences the architecture and gene expression of small intestine tissue in a piglet model

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posted on 2024-02-01, 03:02 authored by Carlos MontoyaCarlos Montoya, Wayne Young, Leigh Ryan, Kelly Dunstan, Jason PetersJason Peters, Hilary DewhurstHilary Dewhurst, James Dekker, Neill Haggarty, Ryan N. Dilger, Nicole C. Roy

This study investigated the effects of Lacticaseibacillus rhamnosus HN001 supplementation on the architecture and gene expression in small intestinal tissues of piglets used as an animal model for infant humans. Twenty-four 10-d-old entire male piglets (4·3 (sd 0·59) kg body weight) were fed an infant formula (IF) (control) or IF supplemented with 1·3 × 105 (low dose) or 7·9 × 106 (high dose) colony-forming units HN001 per ml of reconstituted formula (n 8 piglets/treatment). After 24 d, piglets were euthanised. Samples were collected to analyse the histology and gene expression (RNAseq and qPCR) in the jejunal and ileal tissues, blood cytokine concentrations, and blood and faecal calprotectin concentrations. HN001 consumption altered (false discovery rate < 0·05) gene expression (RNAseq) in jejunal tissues but not in ileal tissues. The number of ileal goblet cells and crypt surface area increased quadratically (P < 0·05) as dietary HN001 levels increased, but no increase was observed in the jejunal tissues. Similarly, blood plasma concentrations of IL-10 and calprotectin increased linearly (P < 0·05) as dietary HN001 levels increased. In conclusion, supplementation of IF with HN001 affected the architecture and gene expression of small intestine tissue, blood cytokine concentration and frequencies, and blood calprotectin concentrations, indicating that HN001 modulated small intestinal tissue maturation and immunity in the piglet model.


New Zealand Ministry of Business, Innovation and Employment (MBIE), contract number C10X1003 ‘Dairy-based solutions for improved intestinal function’

New Zealand Ministry of Business, Innovation and Employment (MBIE), contract number C10X1706 ‘Dairy products for smarter lives’


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© The Author(s), 2023. Published by Cambridge University Press on behalf of The Nutrition Society. This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (, which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.

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  • English

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Cambridge University Press

Journal title

British Journal of Nutrition



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