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The draft genome sequence of the Yersinia entomophaga Entomopathogenic type strain MH96T

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posted on 2023-05-03, 16:47 authored by Mark HurstMark Hurst, Amy BeattieAmy Beattie, Eric Altermann, Roger Moraga, Lincoln A. Harper, Joanne Calder, Aurelie LaugraudAurelie Laugraud
Here we report the draft genome of Yersinia entomophaga type strain MH96T. The genome shows 93.8% nucleotide sequence identity to that of Yersinia nurmii type strain APN3a-cT, and comprises a single chromosome of approximately 4,275,531 bp. In silico analysis identified that, in addition to the previously documented Y. entomophaga Yen-TC gene cluster, the genome encodes a diverse array of toxins, including two type III secretion systems, and five rhs-associated gene clusters. As well as these multicomponent systems, several orthologs of known insect toxins, such as VIP2 toxin and the binary toxin PirAB, and distant orthologs of some mammalian toxins, including repeats-in-toxin, a cytolethal distending toxin, hemolysin-like genes, and an adenylate cyclase, were identified. The genome also contains a large number of hypothetical proteins and orthologs of known effector proteins, such as LopT, as well as genes encoding a wide range of proteolytic determinants, including metalloproteases and pathogen fitness determinants, such as genes involved in iron metabolism. The bioinformatic data derived from the current in silico analysis, along with previous information on the pathobiology of Y. entomophaga against its insect hosts, suggests that a number of these virulence systems are required for hemocoel-based survival and incapacitation of the insect host.

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Rights statement

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

MDPI

Journal title

Toxins

ISSN

2072-6651

Citation

Hurst, M. R. H., Beattie, A., Altermann, E., Moraga, R. M., Harper, L. A., Calder, J., & Laugraud, A. (2016). The draft genome sequence of the Yersinia entomophaga Entomopathogenic type strain MH96T. Toxins, 8(5), 143. doi:10.3390/toxins8050143

Funder

Ministry of Business Innovation & Employment

Contract number

A20199

Job code

294056x04

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