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Target-site and non-target site resistance mechanisms are associated with iodosulfuron resistance in Lolium perenne L.

journal contribution
posted on 2023-06-16, 01:25 authored by Hossein Ghanizadeh, Chris BuddenhagenChris Buddenhagen, Andrew GriffithsAndrew Griffiths, Kerry Harrington, Zachary NgowZachary Ngow

Resistance to iodosulfuron, a herbicide targeting acetolactate synthase (ALS), was identified and characterised in a population of Lolium perenne L. from Canterbury, New Zealand. The resistant population was 264 times more resistant to iodosulfuron than a susceptible population, based on a ratio of the rate corresponding to a 50% reduction in resistant and susceptible plant survival (R/S LD50). The resistant population was also 14-times more resistant to nicosulfuron, another ALS-inhibitor of the same class. However, no resistance to imazapyr, an ALS-inhibitor from a different chemical class, was detected in the resistant population. Pre-treatment with an inhibitor of cytochrome P450 reduced the level of iodosulfuron resistance to 46-fold in the resistant population, suggesting enhanced herbicide metabolism, a non-target site mechanism, contributed to resistance of this herbicide. In addition, a proline to leucine substitution at codon 197 was detected, suggesting a target-site-based mechanism was also associated with resistance to iodosulfuron. This is the first report on the resistance mechanisms to ALS-inhibiting herbicides identified in Lolium perenne in New Zealand and highlights the importance of integrated weed management approaches to reduce the likelihood of ALS-inhibitor herbicide resistance becoming more widespread.

Funding

This research was possible due to the Managing Herbicide Resistance fund (C10X & C10X1806) from the New Zealand Ministry for Business Innovation and Employment

History

Rights statement

© 2022 The Royal Society of New Zealand

Publication date

2022-12-07

Project number

  • Non revenue

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Taylor & Francis

Journal title

New Zealand Journal of Agricultural Research

ISSN

0028-8233

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