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Sucrose assists selection of high-quality oocytes in pigs

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posted on 2023-05-03, 14:24 authored by Thanh Quang Dang‐Nguyen, Hiep Thi Nguyen, Tamas Somfai, David WellsDavid Wells, Nguyen Thi Men, Nguyen Viet‐Linh, Junko Noguchi, Hiroyuki Kaneko, Kazuhiro Kikuchi, Takashi Nagai
We investigated whether high quality in vitro matured (IVM) oocytes can be distinguished from poor ones based on the morphological changes after treatment with hyperosmotic medium containing 0.2 M sucrose in pigs. We hypothesize that IVM oocytes maintaining round shape have higher quality than mis-shapened oocytes following dehydration. Oocyte quality was verified by determining embryonic developmental competence using in vitro fertilization, nuclear transfer and parthenogenetic activation. In all cases, the “round” oocytes had greater (P < 0.05) developmental competence than that of mis-shapened oocytes in terms of blastocyst rate and total cell number in blastocysts obtained after 6 d of in vitro culture. We also confirm that round aged oocytes are higher quality than mis-shapened aged oocytes. In an attempt to find out why high-quality oocytes maintain a round shape whereas poorer oocytes become mis-shapened following sucrose treatment, we examined the arrangement of actin microfilaments and microtubules. Abnormal organization of these cytoskeletal components was higher (P < 0.05) in mis-shapened oocytes compared to round oocytes after 52 h of IVM. In conclusion, sucrose treatment helps selection of high quality oocytes, including aged oocytes, in pigs. Abnormal cytoskeleton arrangements partly explain for low developmental competence of mis-shapened oocytes.

History

Rights statement

© 2018 The Authors. Animal Science Journal published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Animal Science. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Wiley

Journal title

Animal Science Journal

ISSN

1344-3941

Citation

Dang‐Nguyen, T. Q., Nguyen, H. T., Somfai, T., Wells, D., Men, N. T., Viet‐Linh, N., … Nagai, T. (2018). Sucrose assists selection of high-quality oocytes in pigs. Animal Science Journal, 89(6), 880–887. doi:10.1111/asj.13015|

Funder

Royal Society of New Zealand

Contract number

A23750

Job code

15246

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