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Soil nitrogen treatment alters microbiome networks across farm niches

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journal contribution
posted on 2023-05-03, 19:56 authored by XinYue Wang, Kerri ReillyKerri Reilly, Rosemary HeathcottRosemary Heathcott, Ambarish BiswasAmbarish Biswas, Linda JohnsonLinda Johnson, Suliana Teasdale, Gwen-Aelle Grelet, Anastasija Podolyan, Pablo Gregorini, Graeme AttwoodGraeme Attwood, Nik PalevichNik Palevich, Sergio Morales
Agriculture is fundamental for food production, and microbiomes support agriculture through multiple essential ecosystem services. Despite the importance of individual (i.e., niche specific) agricultural microbiomes, microbiome interactions across niches are not well-understood. To observe the linkages between nearby agricultural microbiomes, multiple approaches (16S, 18S, and ITS) were used to inspect a broad coverage of niche microbiomes. Here we examined agricultural microbiome responses to 3 different nitrogen treatments (0, 150, and 300 kg/ha/yr) in soil and tracked linked responses in other neighbouring farm niches (rumen, faecal, white clover leaf, white clover root, rye grass leaf, and rye grass root). Nitrogen treatment had little impact on microbiome structure or composition across niches, but drastically reduced the microbiome network connectivity in soil. Networks of 16S microbiomes were the most sensitive to nitrogen treatment across amplicons, where ITS microbiome networks were the least responsive. Nitrogen enrichment in soil altered soil and the neighbouring microbiome networks, supporting our hypotheses that nitrogen treatment in soil altered microbiomes in soil and in nearby niches. This suggested that agricultural microbiomes across farm niches are ecologically interactive. Therefore, knock-on effects on neighbouring niches should be considered when management is applied to a single agricultural niche.


Rights statement

Copyright © 2022 Wang, Reilly, Heathcott, Biswas, Johnson, Teasdale, Grelet, Podolyan, Gregorini, Attwood, Palevich and Morales. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.


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Journal title

Frontiers in Microbiology




Wang, X., Reilly, K., Heathcott, R., Biswas, A., Johnson, L. J., Teasdale, S., Grelet, G.-A., Podolyan, A., Gregorini, P., Attwood, G. T., Palevich, N., & Morales, S. E. (2022). Soil nitrogen treatment alters microbiome networks across farm niches. Frontiers in Microbiology, 25, 416–422.


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