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EcolEvol2018 Dussex.pdf (809.17 kB)

Reduced representation sequencing detects only subtle regional structure in a heavily exploited and rapidly recolonizing marine mammal species

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posted on 2023-05-03, 13:27 authored by Nicolas Dussex, Helen Taylor, Willam Stovall, Kim Rutherford, Ken Dodds, Shannon ClarkeShannon Clarke, Neil Gemmell
Next‐generation reduced representation sequencing (RRS) approaches show great potential for resolving the structure of wild populations. However, the population structure of species that have shown rapid demographic recovery following severe population bottlenecks may still prove difficult to resolve due to high gene flow between subpopulations. Here, we tested the effectiveness of the RRS method Genotyping‐By‐Sequencing (GBS) for describing the population structure of the New Zealand fur seal (NZFS, Arctocephalus forsteri), a species that was heavily exploited by the 19th century commercial sealing industry and has since rapidly recolonized most of its former range from a few isolated colonies. Using 26,026 neutral single nucleotide polymorphisms (SNPs), we assessed genetic variation within and between NZFS colonies. We identified low levels of population differentiation across the species range (<1% of variation explained by regional differences) suggesting a state of near panmixia. Nonetheless, we observed subtle population substructure between West Coast and Southern East Coast colonies and a weak, but significant (p = 0.01), isolation‐by‐distance pattern among the eight colonies studied. Furthermore, our demographic reconstructions supported severe bottlenecks with potential 10‐fold and 250‐fold declines in response to Polynesian and European hunting, respectively. Finally, we were able to assign individuals treated as unknowns to their regions of origin with high confidence (96%) using our SNP data. Our results indicate that while it may be difficult to detect population structure in species that have experienced rapid recovery, next‐generation markers and methods are powerful tools for resolving fine‐scale structure and informing conservation and management efforts.

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Rights statement

© 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Wiley

Journal title

Ecology and Evolution

ISSN

2045-7758

Citation

Dussex, N., Taylor, H. R., Stovall, W. R., Rutherford, K., Dodds, K. G., Clarke, S. M., & Gemmell, N. J. (2018). Reduced representation sequencing detects only subtle regional structure in a heavily exploited and rapidly recolonizing marine mammal species. Ecology and Evolution, 8(17), 8736–8749. doi:10.1002/ece3.4411