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Plasma biomarkers and identification of resilient metabolic disruptions in patients with venous thromboembolism using a metabolic systems approach

journal contribution
posted on 2023-05-03, 17:48 authored by Karl FraserKarl Fraser, Nicole Roy, Louisa Goumidi, Alexandre Verdu, Pierre Suchon, Felipe Leal-Valentim, David-Alexandre Trégouët, Pierre-Emmanuel Morange, Jean-Charles Martin
Objective: Deep vein thrombosis and pulmonary embolism referred as venous thromboembolism (VTE) are a common cause of morbidity and mortality. Plasma from healthy controls or individuals who have experienced a VTE were analyzed using metabolomics to characterize biomarkers and metabolic systems of VTE patients. Approach and Results: Polar metabolite and lipidomic profiles from plasma collected 3 months after an incident VTE were obtained using liquid chromatography mass spectrometry (LC-MS). Fasting-state plasma samples from 42 patients with venous thromboembolism (VTE) and 42 healthy controls were measured. Plasma metabolomic profiling identified 512 metabolites forming 62 biological clusters. Multivariate analysis revealed a panel of 21 metabolites altogether capable of predicting VTE status with an area under the curve of 0.92 (P=0.00174, selectivity=0.857, sensitivity=0.971). Multiblock systems analysis revealed 25 of the 62 functional biological groups as significantly affected in the VTE group (P<0.05 to control). Complementary correlation network analysis of the dysregulated functions highlighting a subset of the lipidome composed mainly of n-3 long-chain polyunsaturated fatty acids within the predominant triglycerides as a potential regulator of the post-VTE event biological response, possibly controlling oxidative and inflammatory defence systems, and metabolic disorder associated dysregulations. Of interest was microbiota metabolites including trimethylamine N-oxide that remained associated to post incident VTE patients, highlighting a possible involvement of gut microbiota on VTE risk and relapse. Conclusions These findings show promise for the elucidation of underlying mechanisms and the design of a diagnostic test to assess the likely efficacy of clinical care in patients with VTE.


Rights statement

© 2020 American Heart Association, Inc.


  • English

Does this contain Māori information or data?

  • No


American Heart Association, Inc.

Journal title

Arteriosclerosis, Thrombosis, and Vascular Biology




Fraser, K., Roy, N. C., Goumidi, L., Verdu, A., Suchon, P., Leal-Valentim, F., … Martin, J.-C. (2020). Plasma biomarkers and identification of resilient metabolic disruptions in patients with venous thromboembolism using a metabolic systems approach. Arteriosclerosis, Thrombosis, and Vascular Biology, 40, 2527–2538. doi:10.1161/ATVBAHA.120.314480

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