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Ovine rumen papillae biopsy via oral endoscopy: a rapid and repeatable method for serial sampling

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posted on 2023-05-03, 16:34 authored by Kathryn McRaeKathryn McRae, Michael Schultz, Grant ShackellGrant Shackell, Colin Mackintosh, Marcelo Martinez, Kevin Knowler, M Williams, C Ho, Sara Elmes, John McEwanJohn McEwan
AIMS: To explore and validate the utility of rumen endoscopy for collection of rumen papillae for gene expression measurement. METHODS: Four adult Coopworth ewes were fasted for either 4 or 24 hours. Animals were sedated, placed in a dorsally recumbent position at 45 degrees with the head upright, and an endoscope inserted via a tube inserted into the mouth. Biopsies of rumen papillae were taken from the ventral surface of the rumen atrium under visual guidance. Two biopsies were collected from one of the animals that had been fasted for 4 hours, and three from one of the animals that had been fasted for 24 hours. Video of the rumen atrium and reticulum was also collected. The animals recovered uneventfully. Biopsies were subsequently used for extraction and sequencing of mRNA. RESULTS: The ventral surface of the rumen atrium was accessible after 4 hours off pasture, but a larger region was accessible after 24 hours of fasting. Sedation allowed access for endoscope use for around 5 to 10 minutes after which increased saliva flow was noted. Rumen papillae biopsies were easily collected, with samples from a variety of sites collected in the ~10 minute time window. High quality RNA was obtained for stranded mRNA sequencing. Of the resulting reads, 69–70% mapped uniquely to version 3.1 of the ovine genome, and 48–49% to a known gene. The rumen mRNA profiles were consistent with a previously reported study. CONCLUSIONS: This method for obtaining rumenal tissue was found to be rapid and resulted in no apparent short or long term effects on the animal. High quality RNA was successfully extracted and amplified from the rumen papillae biopsies, indicating that this technique could be used for future gene expression studies. The use of rumen endoscopy could be extended to collection of a variety of rumen and reticulum anatomical measurements and deposition and retrieval of small sensors from the rumen. Rumen endoscopy offers an attractive and cost effective approach to repeated rumen biopsies compared with serial slaughter or use of cannulated animals.

History

Rights statement

Taylor & Francis & New Zealand Veterinary Association

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Taylor & Francis & New Zealand Veterinary Association

Journal title

New Zealand Veterinary Journal

ISSN

0048-0169

Citation

McRae, K. M., Schultz, M., Mackintosh C. G., Shackell, G. H., Martinez, M. F., Knowler, K. J., Williams, M., Ho, C., Elmes, S. N., & McEwan, J. C. (2016). Ovine rumen papillae biopsy via oral endoscopy: a rapid and repeatable method for serial sampling. New Zealand Veterinary Journal, 64(3), 174-178. DOI: 10.1080/00480169.2015.1121845

Funder

Ministry of Business Innovation & Employment

Contract number

A20201

Job code

49050X05

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