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Live Faecalibacterium prausnitzii in an apical anaerobic model of the intestinal epithelial barrier

journal contribution
posted on 2023-05-03, 11:00 authored by Dulantha UlluwishewaDulantha Ulluwishewa, Rachel AndersonRachel Anderson, Wayne Young, Warren McNabb, Peter van Baarlen, Paul Moughan, Jerry Wells, Nicole Roy
Faecalibacterium prausnitzii, an abundant member of the human commensal microbiota, has been proposed to have a protective role in the intestine. However, it is an obligate anaerobe, difficult to co-culture in viable form with oxygen-requiring intestinal cells. To overcome this limitation, a unique apical anaerobic model of the intestinal barrier, which enabled co-culture of live obligate anaerobes with the human intestinal cell line Caco-2, was developed. Caco-2 cells remained viable and maintained an intact barrier for at least 12 h, consistent with gene expression data, which suggested Caco-2 cells had adapted to survive in an oxygen-reduced atmosphere. Live F. prausnitzii cells, but not ultraviolet (UV)-killed F. prausnitzii, increased the permeability of mannitol across the epithelial barrier. Gene expression analysis showed inflammatory mediators to be expressed at lower amounts in Caco-2 cells exposed to live F. prausnitzii than UV-killed F. prausnitzii, This, consistent with previous reports, implies that live F. prausnitzii produces an anti-inflammatory compound in the culture supernatant, demonstrating the value of a physiologically relevant co-culture system that allows obligate anaerobic bacteria to remain viable.

History

Rights statement

Copyright © 1999-2015 John Wiley & Sons, Inc. All Rights Reserved.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

John Wiley & Sons, Inc.

Journal title

Cellular Microbiology

ISSN

1462-5814

Citation

Ulluwishewa, D., Anderson, R.C., Young, W., McNabb, W.C., van Baarlen, P., Moughan, P.J., Wells, J.M., & Roy, N.C. (2014). Live faecalibacterium prausnitzii in an apical anaerobic model of the intestinal epithelial barrier. Cellular Microbiology, 17 (2), 226-240

Contract number

A21483

Job code

11352

Report number

FBP 45305

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