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Sengupta_et_al-2015-MicrobiologyOpen.pdf (1.57 MB)

Lactobacillus fermentum AGR1487 cell surface structures and supernatant increase paracellular permeability through different pathways

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posted on 2023-05-03, 12:25 authored by Ranjita Sengupta, Rachel AndersonRachel Anderson, Eric Altermann, Warren McNabb, Siva Ganesh, Kelly Armstrong, Paul Moughan, Nicole Roy
Lactobacillus fermentum is commonly found in food products, and some strains are known to have beneficial effects on human health. However, our previous research indicated that L. fermentum AGR1487 decreases in vitro intestinal barrier integrity. The hypothesis was that cell surface structures of AGR1487 are responsible for the observed in vitro effect. AGR1487 was compared to another human oral L. fermentum strain, AGR1485, which does not cause the same effect. The examination of phenotypic traits associated with the composition of cell surface structures showed that compared to AGR1485, AGR1487 had a smaller genome, utilised different sugars, and had greater tolerance to acid and bile. The effect of the two strains on intestinal barrier integrity was determined using two independent measures of paracellular permeability of the intestinal epithelial Caco-2 cell line. The trans-epithelial electrical resistance (TEER) assay specifically measures ion permeability, whereas the mannitol flux assay measures the passage of uncharged molecules. Both live and UV-inactivated AGR1487 decreased TEER across Caco-2 cells implicating the cell surfaces structures in the effect. However, only live AGR1487, and not UV-inactivated AGR1487, increased the rate of passage of mannitol, implying that a secreted component(s) is responsible for this effect. This difference in barrier integrity results are likely due to the TEER and mannitol flux assays measuring different characteristics of the epithelial barrier, and therefore imply that there are multiple mechanisms involved in the effect of AGR1487 on barrier integrity.

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Rights statement

© 2015 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

John Wiley & Sons Ltd.

Journal title

MicrobiologyOpen

Citation

Sengupta, R., Anderson, R. C., Altermann, E., McNabb, W. C., Ganesh, S., Armstrong, K. M., Moughan, P.J., & Roy, N. C. (2015). Lactobacillus fermentum AGR1487 cell surface structures and supernatant increase paracellular permeability through different pathways. MicrobiologyOpen, 4(4), 541–552. doi:10.1002/mbo3.260

Funder

Ministry of Business Innovation & Employment||Massey University

Contract number

C10X1003

Job code

11351

Report number

FBP 45218

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