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Intragenic Agrobacterium-mediated gene transfer mimics micro-translocations without foreign DNA

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posted on 2024-06-26, 01:29 authored by Philippa Jane Barrell, Julie Latimer, Timothy Millar, Jeanne JacobsJeanne Jacobs, Tony ConnerTony Conner

Intragenic Agrobacterium-mediated gene transfer was achieved in Nicotiana tabacum using a T-DNA composed entirely of N. tabacum DNA, including T-DNA borders and the acetohydroxyacid synthase gene conferring resistance to sulfonylurea herbicides. Genomic analysis of a resulting plant, with single locus inheritance of herbicide resistance, identified a single insertion of the intragenic T-DNA on chromosome 5. The insertion event was composed of three N. tabacum DNA fragments from other chromosomes, as assembled on the T-DNA vector. This validates that intragenic transformation of plants can mimic micro-translocations within genomes, with the absence of foreign DNA.

Funding

New Zealand Foundation for Research, Science and Technology (contract C02X0203)

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Publication date

2024-02-06

Project number

  • Non revenue

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Springer Nature

Journal title

Planta

ISSN

0032-0935

Volume/issue number

259

Page numbers

61

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