AgResearch
Browse
Dalziel Nutrients 2016.pdf (1.63 MB)

Influence of bovine whey protein concentrate and hydrolysate preparation methods on motility in the isolated rat distal colon

Download (1.63 MB)
journal contribution
posted on 2023-05-03, 15:08 authored by Julie DalzielJulie Dalziel, Rachel AndersonRachel Anderson, Shalome Bassett, Catherine Lloyd-West, Neill Haggarty, Nicole Roy
Whey protein concentrate (WPC) and hydrolysate (WPH) are protein ingredients used in sports, medical and pediatric formulations. Concentration and hydrolysis methods vary for whey sourced from cheese and casein co-products. The purpose of this research was to investigate the influence of whey processing methods on in vitro gastrointestinal (GI) health indicators for colonic motility, epithelial barrier integrity and immune modulation. WPCs from casein or cheese processing and WPH (WPH-P; 11% or 19% degree of hydrolysedhydrolysis, DH) and (WPH-AT; 19.4% hydrolysed) were compared for their effects on motility in a 1 cm section of isolated rat distal colon in an oxygenated tissue bath. Results showed that WPC decreased motility irrespective of whether it was a by-product of lactic acid or mineral acid casein production, or from cheese production. This indicated that regardless of the preparation methodology, the whey protein contained components that modulate aspects of motility within the distal colon. WPH-P (11% DH) increased contractile frequency by 27% in a delayed manner and WPH-AT (19% DH) had an immediate effect on contractile properties, increasing tension by 65% and frequency by 131%. Increased motility was associated with increased hydrolysis that may be attributed to the abundance of bioactive peptides. Increased frequency of contractions by WPH-AT (19% DH) was inhibited (by 44%) by naloxone, implicating a potential involvement of opioid receptors in modulation of motility. Trans-epithelial electrical resistance and cytokine expression assays revealed that the whey WPC proteins studied did not alter intestinal barrier integrity or elicit any discernible immune response.

History

Rights statement

© 2016 by the authors; licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

MDPI

Journal title

Nutrients

ISSN

2072-6643

Citation

Dalziel, J. E., Anderson, R. C., Bassett, S. A., Lloyd-West, C. M., Haggarty, N. W., & Roy, N. C. (2016). Influence of bovine whey protein concentrate and hydrolysate preparation methods on motility in the isolated rat distal colon. Nutrients, 8(12), 809. doi:10.3390/nu8120809

Funder

Ministry of Business Innovation & Employment

Contract number

A15883

Job code

11859

Usage metrics

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC