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Inflexibility of the plasma miRNA response following a high-carbohydrate meal in overweight insulin-resistant women

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posted on 2023-05-03, 19:28 authored by Farha Ramzan, Randall D'Souza, Brenan Durainayagam, Amber MilanAmber Milan, Nicole Roy, Marlena Kruger, Christiani Henry, David Cameron-Smith
Context: Metabolic inflexibility is a characteristic of insulin resistance, limiting the ability to transiently regulate oxidative metabolism and gene expression in response to nutrient availability. Little is known of the flexibility of post-transcriptional regulation, including circulatory miRNAs (c-miRNAs). Design: The abundances of targeted c-miRNAs, with reported functions in metabolic regulation, were analysed in response to a high-carbohydrate meal in healthy weight insulin-sensitive (IS) and overweight insulin-resistant (IR) women. Participants: Age-matched healthy weight IS (n = 20, BMI = 24.3 ± 0.70) and overweight IR (n = 20, BMI = 28.6 ± 0.67) women. Methods: An abundance of c-miRNAs was quantified prior to and following a high-carbohydrate breakfast meal (2500 kJ; 50% carbohydrate, 20% fat and 27% protein). Target genes of the differentially regulated c-miRNA were measured in RNA extracted from circulatory peripheral blood mononuclear cells (PBMCs). Results: In healthy weight IS women, both miR-15a-5p (p = 0.03) and miR-17-5p (p < 0.01) levels were halved at 4 h post-meal. These miRNA remained unaltered following the same meal in the overweight IR women. Furthermore, amongst genes targeted by these miRNA, CPT1A (p = 0.01) and IL8 (p = 0.03) had also reduced expression 4 h post-meal only in the healthy weight IS women. Conclusions: The study findings provide preliminary evidence for a possible extension of metabolic inflexibility to include c-miRNAs.

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© The Author(s). 2020 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Springer Nature

Journal title

Genes & Nutrition||Genes & Nutrition

ISSN

1555-8932

Citation

Ramzan, F., D’Souza, R. F., Durainayagam, B. R., Milan, A. M., Roy, N. C., Kruger, M. C., … Cameron-Smith, D. (2020). Inflexibility of the plasma miRNA response following a high-carbohydrate meal in overweight insulin-resistant women. Genes & Nutrition, 15, 2. doi:10.1186/s12263-020-0660-8

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