Genes_Nutr 15_2 - FRamzan et al 040220.pdf (2.19 MB)

Inflexibility of the plasma miRNA response following a high-carbohydrate meal in overweight insulin-resistant women

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posted on 2023-05-03, 19:28 authored by Farha Ramzan, Randall D'Souza, Brenan Durainayagam, Amber MilanAmber Milan, Nicole Roy, Marlena Kruger, Christiani Henry, David Cameron-Smith
Context: Metabolic inflexibility is a characteristic of insulin resistance, limiting the ability to transiently regulate oxidative metabolism and gene expression in response to nutrient availability. Little is known of the flexibility of post-transcriptional regulation, including circulatory miRNAs (c-miRNAs). Design: The abundances of targeted c-miRNAs, with reported functions in metabolic regulation, were analysed in response to a high-carbohydrate meal in healthy weight insulin-sensitive (IS) and overweight insulin-resistant (IR) women. Participants: Age-matched healthy weight IS (n = 20, BMI = 24.3 ± 0.70) and overweight IR (n = 20, BMI = 28.6 ± 0.67) women. Methods: An abundance of c-miRNAs was quantified prior to and following a high-carbohydrate breakfast meal (2500 kJ; 50% carbohydrate, 20% fat and 27% protein). Target genes of the differentially regulated c-miRNA were measured in RNA extracted from circulatory peripheral blood mononuclear cells (PBMCs). Results: In healthy weight IS women, both miR-15a-5p (p = 0.03) and miR-17-5p (p < 0.01) levels were halved at 4 h post-meal. These miRNA remained unaltered following the same meal in the overweight IR women. Furthermore, amongst genes targeted by these miRNA, CPT1A (p = 0.01) and IL8 (p = 0.03) had also reduced expression 4 h post-meal only in the healthy weight IS women. Conclusions: The study findings provide preliminary evidence for a possible extension of metabolic inflexibility to include c-miRNAs.


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© The Author(s). 2020 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.


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Springer Nature

Journal title

Genes & Nutrition||Genes & Nutrition




Ramzan, F., D’Souza, R. F., Durainayagam, B. R., Milan, A. M., Roy, N. C., Kruger, M. C., … Cameron-Smith, D. (2020). Inflexibility of the plasma miRNA response following a high-carbohydrate meal in overweight insulin-resistant women. Genes & Nutrition, 15, 2. doi:10.1186/s12263-020-0660-8

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