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In vitro ileal and caecal fermentation of fibre substrates in the growing pig given a human-type diet

journal contribution
posted on 2023-05-03, 18:48 authored by Carlos MontoyaCarlos Montoya, Paul Blatchford, Paul Moughan
This study characterised the in vitro ileal fermentability of different substrates in the growing pig, adopted as an animal model for the adult human, and compared in vitro ileal and caecal fermentation in the pig. Substrates (arabinogalactan ‘AG’, cellulose, fructooligosaccharide ‘FOS’, inulin, mucin, citrus pectin, and resistant starch) were fermented in vitro (ileal 2 h, caecal 24 h) with an ileal or caecal inoculum prepared from ileal or caecal digesta collected from growing pigs (n=5) fed a human-type diet for 15 days. The organic matter (OM) fermentability and production of organic acids were determined. In general, there was considerable in vitro ileal fermentation of fibre, and the substrates differed (P<0.001) for both in vitro ileal and caecal OM fermentability and for organic acid production. Pectin had the greatest in vitro ileal OM fermentability (26%) followed by AG, FOS, and resistant starch (15% on average), and cellulose, inulin, and mucin (3% on average). The fermentation of FOS, inulin, and mucin was greater for in vitro caecal fermentation compared to the ileal counterpart (P≤0.05). In general, the organic acid production was higher for in vitro caecal fermentation (P≤0.05). For instance, the in vitro ileal acetic acid production represented 4 to 46% of in vitro caecal production. Energy from fibre fermentation of 0.6 to 11 kJ/g substrate fermented could be expected in the ileum of the pig. In conclusion, substrates are fermented in both the ileum and caecum. The degree of fermentation varies among substrates, especially for in vitro ileal fermentation.


Rights statement

© The Authors 2020


  • English

Does this contain Māori information or data?

  • No


Cambridge University Press

Journal title

British Journal of Nutrition




Montoya, C. A., Blatchford, P., & Moughan, P. J. (2020). In vitro ileal and caecal fermentation of fibre substrates in the growing pig given a human-type diet. British Journal of Nutrition, 125(9), 998-1006. doi:10.1017/S0007114520003542

Report number

FBP 90388

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