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In vitro assessment of hydrolysed collagen fermentation using domestic cat (Felis catus) faecal inocula

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posted on 2023-05-03, 21:22 authored by Christina Butowski, David Thomas, Nick Cave, Emma Bermingham, Douglas Rosendale, Shen-Yan Hea, Halina Stoklosinski, Wayne Young
The gastrointestinal microbiome has a range of roles in the host, including the production of beneficial fermentation end products such as butyrate, which are typically associated with fermentation of plant fibres. However, domestic cats are obligate carnivores and do not require carbohydrates. It has been hypothesised that in the wild, collagenous parts of prey—the so-called animal-derived fermentable substrates (ADFS) such as tendons and cartilage—may be fermented by the cat’s gastrointestinal microbiome. However, little research has been conducted on ADFS in the domestic cat. Faecal inoculum was obtained from domestic cats either consuming a high carbohydrate (protein:fat:carbohydrate ratio of 35:20:28 (% dry matter basis)) or high protein (protein:fat:carbohydrate ratio of 75:19:1 (% dry matter basis)) diet. ADFS (hydrolysed collagen, cat hair, and cartilage) were used in a series of static in vitro digestions and fermentations. Concentrations of organic acids and ammonia were measured after 24 h of fermentation, and the culture community of microbes was characterised. The type of inoculum used affected the fermentation profile produced by the ADFS. Butyrate concentrations were highest when hydrolysed collagen was fermented with high protein inoculum (p < 0.05). In contrast, butyrate was not detectable when hydrolysed collagen was fermented in high carbohydrate inoculum (p < 0.05). The microbiome of the domestic cat may be able to ferment ADFS to provide beneficial concentrations of butyrate.

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Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

MDPI

Journal title

Animals

ISSN

2076-2615

Citation

Butowski, C. F., Thomas, D. G., Cave, N. J., Bermingham, E. N., Rosendale, D. I., Hea, S.-Y., Stoklosinski, H. M., & Young, W. (2022). In vitro assessment of hydrolysed collagen fermentation using domestic cat (Felis catus) faecal inocula. Animals, 12(4), 498. https://doi.org/10.3390/ani12040498

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