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Improved detection and fragmentation of disulphide-linked peptides

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posted on 2023-05-03, 20:38 authored by Evelyne MaesEvelyne Maes, Stefan ClerensStefan Clerens, Jolon Dyer, Santanu Deb-Choudhury
Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

MDPI

Journal title

Methods and Protocols

ISSN

2409-9279

Citation

Maes, E., Clerens, S., Dyer, J. M., & Deb-Choudhury, S. (2018). Improved detection and fragmentation of disulphide-linked peptides. Methods and Protocols, 1(3), 33. doi:10.3390/mps1030033

Contract number

A21232

Job code

151802x03

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    Keywords

    MALDI-TOF/TOF MSDisulphide bondsanilineproof-of-existence

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    In Copyright

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