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Impacts of Formula Supplemented with Milk Fat Globule Membrane on the Neurolipidome of Brain Regions of Piglets

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posted on 2023-06-15, 23:26 authored by Karl FraserKarl Fraser, Leigh Ryan, Ryan N. Dilger, Kelly Dunstan, Kelly Armstrong, Jason PetersJason Peters, Hedley Stirrat, Neill Haggerty, Alastair K. H. MacGibbon, James Dekker, Wayne Young, Nicole C. Roy

The milk fat globule membrane (MFGM) appears to play an important role in infant neurocognitive development; however, its mechanism(s) of action remains unclear. This study aimed to investigate the role of a dietary MFGM supplement on the lipid profiles of different neonatal brain regions. Ten-day-old male piglets (4–5 kg) were fed unsupplemented infant formula (control, n = 7) or an infant formula supplemented with low (4%) or high (8%) levels of MFGM (n = 8 each) daily for 21 days. Piglets were then euthanized, and brain tissues were sectioned. Untargeted liquid chromatography-mass spectrometry lipidomics was performed on the cerebellum, hippocampus, prefrontal cortex, and the rest of the brain. The analyses identified 271 and 171 lipids using positive and negative ionization modes, respectively, spanning 16 different lipid classes. MFGM consumption did not significantly alter the lipidome in most brain regions, regardless of dose, compared to the control infant formula. However, 16 triacylglyceride species were increased in the hippocampus (t-test, p-value < 0.05) of the high-supplemented piglets. Most lipids (262 (96.7%) and 160 (93.6%), respectively) differed significantly between different brain regions (ANOVA, false discovery rate corrected p-value < 0.05) independent of diet. Thus, this study highlighted that dietary MFGM altered lipid abundance in the hippocampus and detected large differences in lipid profiles between neonatal piglet brain regions.

Funding

Smarter Lives: New opportunities for dairy products across the lifespan

Ministry of Business, Innovation and Employment

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Rights statement

© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Publication date

2022-07-26

Project number

  • 11600

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

MDPI

Journal title

Metabolites

ISSN

2218-1989

Volume/issue number

12(8)

Page numbers

689

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