AgResearch
Browse
Frontiers Nutr 6_180 - BDurainayagam et al 061219.pdf (2.61 MB)

Impact of a high protein intake on the plasma metabolome in elderly males: 10 week randomized dietary intervention

Download (2.61 MB)
journal contribution
posted on 2023-05-03, 20:25 authored by Brenan Durainayagam, Cameron Mitchell, Amber MilanAmber Milan, Nina Zeng, Pankaja Sharma, Sarah Mitchell, Farha Ramzan, Scott KnowlesScott Knowles, Anders Sjödin, Karl-Heinz Wagner, Nicole Roy, Karl FraserKarl Fraser, David Cameron-Smith
High protein diets may improve the maintenance of skeletal muscle mass in the elderly, although it remains less clear what broader impact such diets have on whole body metabolic regulation in the elderly. Non-targeted polar metabolomics analysis using HILIC HPLC-MS was used to profile the circulating plasma metabolome of elderly men (n = 31; 74.7 ± 4.0 years) who were randomized to consume for 10 weeks a diet designed to achieve either protein (RDA; 0.8·g−1·kg−1) or that doubled this recommend intake (2RDA; 1.6.g.kg−1). A limited number of plasma metabolites (n = 24) were significantly differentially regulated by the diet. These included markers of protein anabolism, which increased by the 2RDA diet, including; urea, creatine, and glutarylcarnitine. Whilst in response to the RDA diet; glutamine, glutamic acid, and proline were increased, relative to the 2RDA diet (p < 0.05). Metaboanalyst identified six major metabolic pathways to be influenced by the quantity of protein intake, most notably the arginine and proline pathways. Doubling of the recommended protein intake in older males over 10 weeks exerted only a limited impact on circulating metabolites, as determined by LC-MS. This metabolomic response was almost entirely due to increased circulating abundances of metabolites potentially indicative of altered protein anabolism, without evidence of impact on pathways for metabolic health.

History

Rights statement

Copyright © 2019 Durainayagam, Mitchell, Milan, Zeng, Sharma, Mitchell, Ramzan, Knowles, Sjödin, Wagner, Roy, Fraser and Cameron-Smith. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Frontiers Media

Journal title

Frontiers in Nutrition

ISSN

2296-861X

Citation

Durainayagam, B., Mitchell, C. J., Milan, A. M., Zeng, N., Sharma, P., Mitchell, S. M., … Cameron-Smith, D. (2019). Impact of a high protein intake on the plasma metabolome in elderly males: 10 week randomized dietary intervention. Frontiers in Nutrition, 6, 180. doi:10.3389/fnut.2019.00180

Contract number

A21246

Usage metrics

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC