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Identification of genetic diversity, pyrrocidine-producing strains and transmission modes of endophytic Sarocladium zeae fungi from Zea crops

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posted on 2023-05-10, 07:47 authored by Qianhe LiuQianhe Liu, Linda JohnsonLinda Johnson, Emma Applegate, Karoline Arfmann, Ruy Jauregui, Anna LarkingAnna Larking, Wade MaceWade Mace, Paul MacleanPaul Maclean, Thomas Walker, Richard JohnsonRichard Johnson
Genotyping by sequencing (GBS) was used to reveal the inherent genetic variation within the haploid fungi Sarocladium zeae isolated from diverse Zea germplasm, including modern Zea mays and its wild progenitors—the teosintes. In accordance with broad host relationship parameters, GBS analysis revealed significant host lineages of S. zeae genetic diversity, indicating that S. zeae genetic variation may associate with different evolutionary histories of host species or varieties. Based on a recently identified PKS-NRPS gene responsible for pyrrocidine biosynthesis in S. zeae fungi, a novel PCR assay was developed to discriminate pyrrocidine-producing S. zeae strains. This molecular method for screening bioactive strains of S. zeae is complementary to other approaches, such as chemical analyses. An eGFP-labelled S. zeae strain was also developed to investigate the endophytic transmission of S. zeae in Z. mays seedlings, which has further improved our understanding of the transmission modes of S. zeae endophytes in maize tissues.

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Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

MDPI

Journal title

Microorganisms

ISSN

2076-2607

Citation

Liu, Q., Johnson, L. J., Applegate, E. R., Arfmann, K., Jauregui, R., Larking, A., Mace, W. J., Maclean, P., Walker, T., & Johnson, R. D. (2022). Identification of genetic diversity, pyrrocidine-producing strains and transmission modes of endophytic Sarocladium zeae fungi from Zea crops. Microorganisms, 10(7), 1415. https://doi.org/10.3390/microorganisms10071415

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