AgResearch
Browse
Identification Umairl.pdf (413.72 kB)

Identification of Peptide Mimics of a Glycan Epitope on the Surface of Parasitic Nematode Larvae

Download (413.72 kB)
journal contribution
posted on 2023-05-03, 12:49 authored by Saleh UmairSaleh Umair, Qing DengQing Deng, Joanna Roberts, Richard ShawRichard Shaw, Ian SutherlandIan Sutherland, Tony Pernthaner
Phage display was used to identify peptide mimics of an immunologically protective nematode glycan (CarLA) by screening a constrained C7C peptide library for ligands that bound to an anti-CarLA mAb (PAB1). Characterisation of these peptide mimotopes revealed functional similarities with an epitope that is defined by PAB1. Mimotope vaccinations of mice with three selected individual phage clones facilitated the induction of antibody responses that recognised the purified, native CarLA molecule which was obtained from Trichostrongylus colubriformis. Furthermore, these mimotopes are specifically recognised by antibodies in the saliva of animals that were immune to natural polygeneric nematode challenge. This shows that antibodies to the PAB1 epitope form part of the mucosal polyclonal anti- CarLA antibody response of nematode immune host animals. This demonstrates that the selected peptide mimotopes are of biological relevance. These peptides are the first to mimic the PAB1 epitope of CarLA, a defined larval glycan epitope which is conserved between many nematode species.

History

Rights statement

© 2016 Umair et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

PLOS

Journal title

PLoS ONE

ISSN

1932-6203

Citation

Umair, S., Deng, Q., Roberts, J. M., Shaw, R. J., Sutherland, I. A., & Pernthaner, A. (2016). Identification of peptide mimics of a glycan epitope on the surface of parasitic nematode larvae. PLoS ONE, 11(8), e0162016. doi:10.1371/journal.pone.0162016

Usage metrics

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC