AgResearch
Browse

File(s) not publicly available

Histological methods to detect early-stage plant defence responses during artificial inoculation of Lolium perenne with Epichloë festucae

journal contribution
posted on 2023-05-10, 07:47 authored by Mostafa Rahnama, Damien Fleetwood, Richard JohnsonRichard Johnson
Epichloë species form agriculturally important symbioses with many cool season grasses. To study these symbioses, such as the interaction of Epichloë festucae with perennial ryegrass (Lolium perenne), host plants can be infected by artificial inoculation of etiolated seedlings. This inoculation is performed by placing mycelium into an incision in the meristem, as previously described by Latch and Christensen (1985). In recent years, this method has been broadly used to study this interaction at the molecular level using different Epichloë festucae mutants that can cause incompatible interactions. We have developed and adapted methods to study four of the most important host plant responses to infection, including cell death, callose deposition, lignin production, and hydrogen peroxide (H2O2) production, which are useful in defining the host response to infection at a very early time point.

History

Rights statement

Copyright: © 2021 The Authors; exclusive licensee Bio-protocol LLC.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Bio-protocol LLC

Journal title

Bio-protocol

ISSN

2331-8325

Citation

Rahnama, M., Fleetwood, D. J., & Johnson, R. D. (2021). Histological methods to detect early-stage plant defence responses during artificial inoculation of Lolium perenne with Epichloë festucae. Bio-protocol, 11(9), e4013. https://doi.org/10.21769/BioProtoc.4013

Usage metrics

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC