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Elevation of condensed tannins in the leaves of Ta-MYB14-1 white clover (Trifolium repens L.) outcrossed with high anthocyanin lines

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posted on 2023-05-03, 21:36 authored by Marissa RoldanMarissa Roldan, Greig Cousins, Karl FraserKarl Fraser, Kerry Hancock, Vern Collette, Jerome Demmer, Derek Woodfield, John CaradusJohn Caradus, Chris Jones, Christine VoiseyChristine Voisey
Condensed tannins (CT) are highly desirable in forage as they sequester dietary protein, reducing bloat and methane emissions in ruminants. However, the widely used forage legume white clover (Trifolium repens) only produces CTs in flowers and trichomes, and at levels too low to achieve therapeutic effects. Genetic transformation with transcription factor Ta-MYB14-1 from T. arvense was effective in inducing CTs to 0.6% of leaf dry matter. CT synthesis has been elevated further by crossing the primary white clover transgenic line with wild type genotypes producing the related phenylpropanoids, anthocyanins. CT levels in leaves were highest under the anthocyanin leaf marks associated with the ‘red midrib’ traits, however there was no evidence for CT accumulation in leaf sections with the “red V” anthocyanin marking. Ta-MYB14-1 was stably inherited in two generations of crosses, and T2 progeny produced up to 3.6-fold higher CTs than the T0 parent. The profile of small CT oligomers such as dimers and trimers was consistent in T0, T1, T2 and BC2 progeny and consisted predominantly of prodelphinidins (PD), with lesser amounts of procyanidins (PC) and mixed PC:PD oligomers.

History

Rights statement

Copyright © 2019 American Chemical Society

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

ACS Publications

Journal title

Journal of Agricultural and Food Chemistry

ISSN

0021-8561

Citation

Roldan, M. B., Cousins, G., Fraser, K., Hancock, K., Collette, V., Demmer, J., … Voisey, C. (2020). Elevation of condensed tannins in the leaves of Ta-MYB14-1 white clover (Trifolium repens L.) outcrossed with high anthocyanin lines. Journal of Agricultural and Food Chemistry, 68(10), 2927–2939. doi:10.1021/acs.jafc.9b01185

Funder

Ministry for Primary Industries||Grasslanz Technology||PGG Wrightson

Contract number

A19765

Job code

50168

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