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Differential expression of CART in ewes with differing ovulation rates

journal contribution
posted on 2023-05-03, 11:35 authored by Jenny JuengelJenny Juengel, Michelle FrenchMichelle French, Laurel QuirkeLaurel Quirke, Alexia Kauff, George Smith, Peter Johnstone
We hypothesised that cocaine- and amphetamine-regulated transcript (CARTPT) would be differentially expressed in ewes with differing ovulation rates. Expression of mRNA for CARTPT, as well as LHCGR, FSHR, CYP19A1 and CYP17A1 was determined in antral follicles ≥1 mm in diameter collected during the follicular phase in ewes heterozygous for the Booroola and Inverdale genes (I+B+; average ovulation rate 4) and ++ contemporaries (++; average ovulation rate 1.8). In ++ ewes (n = 6), CARTPT was expressed in small follicles (1 to <3 mm diameter), where 18.8 ± 2.5% follicles expressed CARTPT. CART peptide was also detected in follicular fluid of some follicles of ++ ewes. In I+B+ ewes, 5/6 ewes did not have any follicles that expressed CARTPT, and no CART peptide was detected in any follicle examined. Expression pattern of CYP19A1 differed between I+B+ and ++ ewes with an increased percentage of small and medium follicles (3 to <4.5 mm diameter) but decreased percentage of large follicles (≥4.5 mm diameter) expressing CYP19A1 in the I+B+ ewes. Many of the large follicles from the I+B+ ewes appeared non-functional and expression of LHCGR, FSHR, CYP17A1 and CYP19A1 was less than that observed in ++ ewes. Expression of FSHR and CYP17A1 was not different between groups in small and medium follicles, but LHCGR expression was approximately double in I+B+ ewes compared to that in ++ ewes. Thus, ewes with high ovulation rates had a distinct pattern of expression of CARTPT mRNA and protein compared to ewes with normal ovulation rates, providing evidence for CART being important in the regulation of ovulation rate.

History

Rights statement

Copyright © 2017 by the Society for Reproduction and Fertility

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Society for Reproduction and Fertility

Journal title

Reproduction

ISSN

1470-1626

Citation

Juengel, J. L., French, M. C., Quirke, L. D., Kauff, A., Smith, G. W., & Johnstone, P. D. (2017). Differential expression of CART in ewes with differing ovulation rates. Reproduction, 153, 471–479. doi:10.1530/REP-16-0657

Funder

Marsden Fund

Contract number

A17616

Job code

31146

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