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Current understanding on the heterogenous expression of de-polymerizing enzymes in Pichia pastoris

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posted on 2025-01-23, 19:59 authored by Shuyan WuShuyan Wu, David HooksDavid Hooks, Gale BrightwellGale Brightwell

Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation. Microbial heterologous expression systems have been studied for meeting this demand. Among these systems, the Pichia pastoris expression system has emerged as a widely used platform for producing secreted heterologous proteins. This article provides an overview of studies involving the recombinant expression of polymer-degrading enzymes using the P. pastoris expression system. Research on P. pastoris expression of interested enzymes with depolymerising ability, including cutinase, lipase, and laccase, are highlighted in the review. The key factors influencing the heterologous expression of polymer-degrading enzymes in P. pastoris are discussed, shedding light on the challenges and opportunities in the development of depolymerising biocatalysts through the P. pastoris expression system.

Funding

AgResearch Strategic Science Investment Fund

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Rights statement

© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Publication date

2025-01-14

Project number

  • PRJ0760857

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

MDPI

Journal title

Bioengineering

ISSN

2306-5354

Volume/issue number

12(1)

Page numbers

68

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