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Creation of DNA aptamers against recombinant bone morphogenetic protein 15

journal contribution
posted on 2023-05-10, 08:06 authored by J. Lin, Alexia Kauff, Yong Diao, Huiyong Yang, Steve Lawrence, Jenny JuengelJenny Juengel
The oocyte-derived growth factor bone morphogenetic protein (BMP) 15 plays important roles in fertility, but its mechanism of action differs between species. Generation of BMP15-binding molecules, as an essential investigation tool, would be helpful to provide valuable insight into the underlying biological features of BMP15. The BMP15-binding molecules could be antibodies or aptamers. Aptamers have many advantages over antibodies as macromolecular ligands for target proteins. DNA aptamers can be obtained by a method of Systematic Evolution of Ligands by EXponential enrichment (SELEX) beginning with a pool of random sequences. However, the success of this technique cannot be guaranteed if the initial pool lacks candidate sequences. Herein, we report on the creation of DNA aptamers by means of modified SELEX. The modification included enhanced mutation and progressive selection during an in vitro evolutionary process. As a proof-of-principle, we started from a single sequence instead of a multiple-sequence pool. Functional aptamers against the recombinant BMP15 were successfully created and identified.

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Rights statement

© CSIRO 2014

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

CSIRO

Journal title

Reproduction, Fertility and Development

ISSN

1031-3613

Citation

Lin, J. S., Kauff, A., Diao, Y., Yang, H., Lawrence, S., & Juengel, J. L. (2015). Creation of DNA aptamers against recombinant bone morphogenetic protein 15. Reproduction, Fertility and Development, 28(8), 1164-1171. doi: 10.1071/RD14409

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