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Metabolites 9(9)_189 - MAgnew et al 180919.pdf (2.36 MB)

Comprehensive evaluation of parameters affecting one-step method for quantitative analysis of fatty acids in meat

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Despite various direct transmethylation methods having been published and applied to analysis of meat fatty acid (FA) composition, there are still conflicting ideas about the best method for overcoming all the difficulties posed by analysis of complex mixtures of FA in meat. This study performed a systematic investigation of factors affecting a one-step method for quantitative analysis of fatty acids in freeze-dried animal tissue. Approximately 280 reactions, selected using factorial design, were performed to investigate the effect of temperature, reaction time, acid concentration, solvent volume, sample weight and sample moisture. The reaction yield for different types of fatty acids, including saturated, unsaturated (cis, trans and conjugated) and long-chain polyunsaturated fatty acids was determined. The optimised condition for one-step transmethylation was attained with four millilitres 5% sulfuric acid in methanol (as acid catalyst), four millilitres toluene (as co-solvent), 300 mg of freeze-dried meat and incubation at 70 °C for 2 h, with interim mixing by inversion at 30, 60 and 90 min for 15 s. The optimised condition was applied to meat samples from different species, covering a broad range of fat content and offers a simplified and reliable method for analysis of fatty acids from meat samples.

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Rights statement

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Publication date

2019-09-18

Project number

  • Non revenue

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

MDPI

Journal title

Metabolites

ISSN

2218-1989

Citation

Agnew, M. P., Craigie, C. R., Weralupitiya, G., Reis, M. M., Johnson, P. L., & Reis, M. G. (2019). Comprehensive evaluation of parameters affecting one-step method for quantitative analysis of fatty acids in meat. Metabolites, 9(9), 189. doi:10.3390/metabo9090189

Funder

Strategic Science Investment Fund (SSIF)

Contract number

A22713

Job code

14485x01

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