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Circulatory miRNA biomarkers of metabolic syndrome

journal contribution
posted on 2023-05-03, 19:13 authored by Farha Ramzan, Randall D'Souza, Brenan Durainayagam, Amber MilanAmber Milan, James Markworth, Victor Miranda-Soberanis, Ivana Sequeira, Nicole Roy, Sally Poppitt, Cameron Mitchell, David Cameron-Smith
AIMS: Circulatory microRNAs (c-miRNAs) exert important roles in the molecular dysregulation of cardio-metabolic diseases. However, little is known whether dysregulated miRNA expression occurs when risk factors are elevated, as in the metabolic syndrome (MetS). This study quantified c-miRNA expression in individuals with MetS compared to healthy, further examining the relationship of gene pathways with the underlying pathogenesis. METHODS: Expression of 26 miRNAs was quantified in plasma from 40 women (20 healthy and 20 MetS) and 39 men (20 healthy and 19 MetS) by qPCR. In silico analysis was performed to investigate biological effects of the dysregulated miRNAs. Dysregulated miRNA expression was further validated in an independent cohort of 20 women (10 healthy and 10 MetS). RESULTS: Regression model adjusted for age and sex identified miR-15a-5p, miR-17-5p, miR-370-3p and miR-375 as important predictors of MetS presence. Analysis of predictive miRNAs in the validation cohort strengthened the relationship with miR-15a-5p and miR-17-5p expression. These miRNAs share genes involved in the regulation of metabolic pathways including insulin, wnt, fatty acid metabolism and AMPK. CONCLUSIONS: miR-15a-5p and miR-17-5p were identified as predictive biomarkers of MetS, irrespective of sexes, further demonstrating the relationship of c-miRNAs to known pathways of metabolic disturbances present in cardio-metabolic diseases.

History

Rights statement

© Springer-Verlag Italia S.r.l., part of Springer Nature 2019

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Springer Nature

Journal title

Acta Diabetologica

ISSN

0940-5429

Citation

Ramzan, F., D’Souza, R. F., Durainayagam, B. R., Milan, A. M., Markworth, J. F., Miranda-Soberanis, V., … Cameron-Smith, D. (2020). Circulatory miRNA biomarkers of metabolic syndrome. Acta Diabetologica, 57, 203–214. doi:10.1007/s00592-019-01406-6

Contract number

A21246

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