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Butyrivibrio hungatei MB2003 competes effectively for soluble sugars released by Butyrivibrio proteoclasticus B316T from growth on xylan or pectin.

journal contribution
posted on 2023-05-03, 15:28 authored by Nik PalevichNik Palevich, William Kelly, Siva Ganesh, Jasna Rakonjac, Graeme AttwoodGraeme Attwood
Rumen bacterial species belonging to the genera Butyrivibrio are important degraders of plant polysaccharides, particularly hemicelluloses (arabinoxylans) and pectin. Currently, four distinct species are recognized which have very similar substrate utilization profiles, but little is known about how these microorganisms are able to co-exist in the rumen. To investigate this question, Butyrivibrio hungatei (MB2003) and Butyrivibrio proteoclasticus (B316T) were grown alone or in co-culture on the insoluble substrates, xylan or pectin, and their growth, release of sugars, fermentation end products and transcriptomes were examined. In single cultures, B316T was able to degrade and grow well on xylan and pectin, while B. hungatei MB2003 was unable to utilize either of these insoluble substrates to support significant growth. Co-cultures of B316T grown with MB2003 revealed that MB2003 showed almost equivalent growth to B316T when either xylan or pectin were supplied as substrates. The effect of co-culture on the transcriptomes of B316T and MB2003 was very marked; B316T transcription was largely unaffected by the presence MB2003, but MB2003 expressed a wide range of genes encoding carbohydrate degradation/metabolism and oligosaccharide transport/assimilation in order to compete with B316T for the released sugars. These results suggest that B316T has a role as an initiator of the primary solubilization of xylan and pectin, while MB2003 competes effectively as a scavenger for the released soluble sugars to enable its growth and maintenance in the rumen.

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Rights statement

Copyright © 2019 Palevich et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

American Society for Microbiology

Journal title

Applied and Environmental Microbiology

ISSN

0099-2240

Citation

Palevich, N., Kelly, W. J., Ganesh, S., Rakonjac, J., & Attwood, G. T. (2019). Butyrivibrio hungatei MB2003 competes effectively for soluble sugars released by Butyrivibrio proteoclasticus B316T from growth on xylan or pectin. Applied and Environmental Microbiology, 85, e02056–18. doi:10.1128/AEM.02056-18

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