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Bioengineering a bacterial pathogen to assemble its own particulate vaccine capable of inducing cellular immunity

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posted on 2023-05-03, 10:43 authored by Jason Lee, Natalie ParlaneNatalie Parlane, Neil WedlockNeil Wedlock, Bernd Rehm
Many bacterial pathogens naturally form cellular inclusions. Here the immunogenicity of polyhydroxyalkanoate (PHA) inclusions and their use as particulate vaccines delivering a range of host derived antigens was assessed. Our study showed that PHA inclusions of pathogenic Pseudomonas aeruginosa are immunogenic mediating a specific cell-mediated immune response. Protein engineering of the PHA inclusion forming enzyme by translational fusion of epitopes from vaccine candidates outer membrane proteins OprI, OprF, and AlgE mediated self-Assembly of PHA inclusions coated by these selected antigens. Mice vaccinated with isolated PHA inclusions produced a Th1 type immune response characterized by antigen-specific production of IFN-3 and IgG2c isotype antibodies. This cell-mediated immune response was found to be associated with the production of functional antibodies reacting with cells of various P. aeruginosa strains as well as facilitating opsonophagocytic killing. This study showed that cellular inclusions of pathogenic bacteria are immunogenic and can be engineered to display selected antigens suitable to serve as particulate subunit vaccines against infectious diseases.

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This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Springer Nature

Journal title

Scientific Reports

ISSN

2045-2322

Citation

Lee, J. W., Parlane, N. A., Wedlock, D. N., & Rehm, B. H. A. (2017). Bioengineering a bacterial pathogen to assemble its own particulate vaccine capable of inducing cellular immunity. Scientific Reports, 7, 41607. doi:10.1038/srep41607

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