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Anisotropic light propagation in bovine muscle tissue depends on the initial fiber orientation, muscle type and wavelength

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journal contribution
posted on 2023-05-03, 10:33 authored by Robbe Van Beers, Ben Aernouts, Marlon M.Reis, Wouter Saeys
The effects of fiber orientation on vis/NIR light propagation were studied in three bovine muscles: biceps brachii, brachialis and soleus. Broadband light was focused onto the sample and the diffuse reflectance spot was captured using a hyperspectral camera (470-1620 nm), after which rhombuses were fitted to equi-intensity points. In samples with fibers running parallel to the measurement surface, the rhombus’ major axis was oriented perpendicular to the fiber direction close to the point of illumination. However, at larger distances from the illumination spot, the major axis orientation aligned with the fiber direction. This phenomenon was found to be muscle dependent. Furthermore, the rhombus orientation was highly dependent on the sample positioning underneath the camera, especially when the muscle fibers ran parallel to the measurement surface. The bias parameter, indicating the deviation from a circular shape, was higher for samples with the fibers running parallel to the measurement surface. Moreover, clear effects of wavelength and distance from the illumination point on this parameter were observed. These results show the importance of fiber orientation when considering optical techniques for measurements on anisotropic, fibrous tissues. Moreover, the prediction of muscle fiber orientation seemed feasible, which can be of interest to the meat industry.

History

Rights statement

© 2017 Optical Society of America

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

OSA Publishing

Journal title

Optics Express

ISSN

1094-4087

Citation

Van Beers, R., Aernouts, B., M.Reis, M., & Saeys, W. (2018). Anisotropic light propagation in bovine muscle tissue depends on the initial fiber orientation, muscle type and wavelength. Optics Express, 25(18), 22082–22095. doi:10.1364/OE.25.022082

Report number

FBP 78134