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Analysis of human faecal host proteins: responsiveness to 10-week dietary intervention modifying dietary protein intake in elderly males

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posted on 2023-05-03, 18:32 authored by Jessica Gathercole, Anita Grosvenor, Erin LeeErin Lee, Ancy ThomasAncy Thomas, Cameron Mitchell, Nina Zeng, Randall D'Souza, Farha Ramzan, Pankaja Sharma, Scott KnowlesScott Knowles, Nicole Roy, Anders Sjödin, Karl-Heinz Wagner, Amber MilanAmber Milan, Sarah Mitchell, David Cameron-Smith
Faecal proteomics targeting biomarkers of immunity and inflammation have demonstrated clinical application for the identification of changes in gastrointestinal function. However, there are limited comprehensive analyses of the host faecal proteome and how it may be influenced by dietary factors. To examine this, the Homo sapiens post-diet proteome of older males was analysed at the completion of a 10-week dietary intervention, either meeting the minimum dietary protein recommendations (RDA; n = 9) or twice the recommended dietary allowance (2RDA, n = 10). The host faecal proteome differed markedly between individuals, with only a small subset of proteins present in ≥ 60% of subjects (14 and 44 proteins, RDA and 2RDA, respectively, with only 7 common to both groups). No differences were observed between the diet groups on the profiles of host faecal proteins. Faecal proteins were detected from a wide range of protein classes, with high inter-individual variation and absence of obvious impact in response to diets with markedly different protein intake. This suggests that well-matched whole food diets with two-fold variation in protein intake maintained for 10 weeks have minimal impact on human faecal host proteins.


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Copyright © 2021 Gathercole, Grosvenor, Lee, Thomas, Mitchell, Zeng, D'Souza, Ramzan, Sharma, Knowles, Roy, Sjödin, Wagner, Milan, Mitchell and Cameron-Smith. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.


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Frontiers Media

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Frontiers in Nutrition




Gathercole, J. L., Grosvenor, A. J., Lee, E., Thomas, A., Mitchell, C. J., Zeng, N., … Cameron-Smith, D. (2021). Analysis of human faecal host proteins: responsiveness to 10-week dietary intervention modifying dietary protein intake in elderly males. Frontiers in Nutrition, 7, 595905. doi:10.3389/fnut.2020.595905

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