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A large-scale metabolomics study to harness chemical diversity and explore biochemical mechanisms in ryegrass

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posted on 2023-05-03, 19:40 authored by Arvind SubbarajArvind Subbaraj, Jan Huege, Karl FraserKarl Fraser, Mingshu CaoMingshu Cao, Susanne Rasmussen, Marty FavilleMarty Faville, Scott Harrison, Chris Jones
Perennial ryegrass (Lolium perenne) is integral to temperate pastoral agriculture, which contributes most of the milk and meat production worldwide. Chemical profiles and diversity of ryegrass offer several opportunities to harness specific traits and elucidate underlying biological mechanisms for forage improvement. We conducted a large-scale metabolomics study of perennial ryegrass comprising 715 genotypes, representing 118 populations from 21 countries. Liquid/gas chromatography–mass spectrometry based targeted and non-targeted techniques were used to analyse fructan oligosaccharides, lipids, fatty acid methyl esters, polar and semi-polar compounds. Fructan diversity across all genotypes was evaluated, high- and low-sugar groups identified, and fructan accumulation mechanisms explored. Metabolites differentiating the two groups were characterised, modules and pathways they represent deduced, and finally, visualisation and interpretation provided in a biological context. We also demonstrate a workflow for large-scale metabolomics studies from raw data through to statistical and pathway analysis. Raw files and metadata are available at the MetaboLights database.


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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.


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Springer Nature

Journal title

Communications Biology




Subbaraj, A., Huege, J., Fraser, K., Cao, M., Rasmussen, S., Faville, M., … Jones, C. (2019). A large-scale metabolomics study to harness chemical diversity and explore biochemical mechanisms in ryegrass. Communications Biology, 2, 87. doi:10.1038/s42003-019-0289-6

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