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Role of pH and nutrient source on the production and release of aucubin, acteoside and catalpol in hydroponically grown Plantago lanceolata

conference contribution
posted on 2024-06-05, 02:14 authored by Priscila Simon, Soledad Navarrete, Arvind SubbarajArvind Subbaraj, Cecile de KleinCecile de Klein, Marta Alfaro ValenzuelaMarta Alfaro Valenzuela

Nitrous oxide (N2O) emissions contribute c. 20% of total agricultural greenhouse gas (GHG) emissions in New Zealand. The incorporation of plantain (Plantago lanceolata) in pastures has reportedly reduced N2O emissions through the production of secondary compounds inducing biologically nitrification inhibition (BNI), although the mechanisms associated to their release are still unclear. The experiment aimed to understand the role of pH (4.2, 5.6 and 6.8) and nitrogen (N) source (N-NH4+, N-NO3-) on plantain’s BNI metabolites. For this, plantain and ryegrass (Lolium perenne, control) were sown in rockwool cubes and after 10 days the seedlings were transferred to a hydroponic system for a period of 45 days (30 L cubic tanks, 8 plants per tank), solution N concentration and pH were revised and adjusted weekly. An additional solution medium was used to collect root exudates. The concentration of aucubin, acteoside and catalpol in root exudates, leaf and root samples were determined by high-performance liquid chromatography (HPLC) and UV-Vis detector (leaf and root) or a mass spectrometer (root exudates), and data was analysed using SAS 9.6. All three metabolites were found in higher concentrations in plantain leaves and roots, in comparison to ryegrass (p<0.05). Higher concentrations of acteoside were found at pH 5.6 (0.51±0.008 mg/g DM for pH 5.6 and 0.45±0.013 mg/g DM for pH 4.2 and 6.8, on average, respectively; p<0.07), and when N-NH4+ was used as N source (0.73±0.007 mg mg/g DM for NH4+ and 0.40±0.03 mg mg/g DM for NO3-1 treatments, respectively; p<0.05), reflecting that weakly acid conditions may favour the release of this metabolite. No significant differences between treatments were found for aucubin concentrations in plant tissue (p>0.05), and catalpol was not detected in leaves or roots. Aucubin, catapol and acteoside were not detected in root exudates, with implications for the detection methodology.

Funding

Centre for Climate Action on Agricultural Emissions of the Ministry for Primary Industries

History

Rights statement

This is an open-access output. It may be used, distributed or reproduced in any medium, provided the original author and source are credited.

Publication date

2024-05-13

Project number

  • PRJ0648681

Language

  • English

Does this contain Māori information or data?

  • No

Conference name

Centennial Celebration and Congress of the International Union of Soil Sciences

Conference location

Florence, Italy

Conference start date

2024-05-19

Conference end date

2024-05-21

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