SQGSeattle2023-Dodds.pdf (1.69 MB)
Allowing for non-binomial sampling of alleles in sequencing-based genotypes
conference contribution
posted on 2024-01-15, 20:08 authored by Ken DoddsKen Dodds, Alan McCulloch, Rudiger BrauningRudiger Brauning, Tracey Van StijnTracey Van Stijn, Michael Bates, Shannon ClarkeShannon ClarkeOutline
- Analysis of Genotyping By Sequencing (GBS) data
- How can we detect insufficient deduplication without “gold-standard” inbreeding estimates?
- Can we make adjustments later in the analysis process (without flowcell position information)?
Conclusions
- Deduplication is a useful tool with patterned flowcells
- But parameter settings unclear
- Using non-binomial allele sampling models can correct remaining examp duplication effects
- Suitable training sets needed to parametrize models
- Assaying libraries across 2 lanes of a flowcell provides suitable data
History
Rights statement
This is an open-access output. It may be used, distributed or reproduced in any medium, provided the original author and source are credited.Publication date
2023-07-14Project number
- 49050
Language
- English
Does this contain Māori information or data?
- No
