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Purification of the Yersinia entomophaga Yen-TC toxin complex using size exclusion chromatography

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posted on 2023-05-03, 14:39 authored by Sandra Jones, Mark HurstMark Hurst
The Yersinia entomophaga toxin complex (Yen-TC) is the bacterium’s main virulence determinant. Because of its high insect activity, methods were developed to allow the routine isolation and purification of Yen-TC from an overnight bacterial culture. Here we outline an overnight purification procedure using a 100-ml culture volume, where approximately 2 mg of Yen-TC, with an approximate purity of 95–98%, can be routinely obtained.

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Rights statement

© Springer International Publishing AG. Springer Science+Business Media New York

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Springer Nature

Journal title

Microbial-based biopesticides: methods and protocols

ISSN

1064-3745

ISBN

9781493963652

Citation

Jones, S. A., & Hurst, M. R. H. (2016). Purification of the Yersinia entomophaga Yen-TC toxin complex using size exclusion chromatography. In T. R. Glare & M. E. Moran-Diez (Eds.), Microbial-based biopesticides: methods and protocols. Methods in Molecular Biology, Vol. 1477 (pp. 39–48). doi:10.1007/978-1-4939-6367-6_4

Funder

Ministry of Business Innovation & Employment

Contract number

A20199

Job code

294056

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