The Yersinia entomophaga toxin complex (Yen-TC) is the bacterium’s main virulence determinant. Because of its high insect activity, methods were developed to allow the routine isolation and purification of Yen-TC from an overnight bacterial culture. Here we outline an overnight purification procedure using a 100-ml culture volume, where approximately 2 mg of Yen-TC, with an approximate purity of 95–98%, can be routinely obtained.
Microbial-based biopesticides: methods and protocols
ISSN
1064-3745
ISBN
9781493963652
Citation
Jones, S. A., & Hurst, M. R. H. (2016). Purification of the Yersinia entomophaga Yen-TC toxin complex using size exclusion chromatography. In T. R. Glare & M. E. Moran-Diez (Eds.), Microbial-based biopesticides: methods and protocols. Methods in Molecular Biology, Vol. 1477 (pp. 39–48). doi:10.1007/978-1-4939-6367-6_4