AgResearch
Browse

In vitro culture of deer embryos

Download (17.6 kB)
chapter
posted on 2023-05-03, 19:04 authored by Debbie BergDebbie Berg, Ampika Thongphakdee
In vitro embryo production of deer species has the potential to increase valuable traits for the agricultural sector, and from a conservation prospective, it is a propagation tool which can improve genetic diversity in small captive populations. In vitro embryo production is a multi-step process consisting of oocyte maturation, fertilization and embryo culture. These techniques provide the backbone for more advanced assisted reproductive technologies such as intracytoplasmic sperm injection (ICSI), somatic cell nuclear transfer (SCNT), a source of embryonic stem cells, and embryos for gene editing. In vitro produced embryos are a readily available resource for comparative embryology studies and a functional assay to assess oocyte competence and evaluate in vitro embryo requirements during culture. A semi-defined fertilization and culture medium system, Deer Synthetic Oviduct Fluid (DSOF), has been formulated based on deer oviduct fluid. Red deer calves (Cervus elaphus) and Thamin Eld’s deer fawn (Rucervus eldii thamin) have been produced after the transfer of in vitro embryos (IVF and SCNT) grown in DSOF culture. Here we describe the in vitro method of maturation, fertilization and embryo culture for deer species.

History

Rights statement

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Springer Nature

Journal title

Comparative embryo culture: methods and protocols

ISBN

9781493995653

Citation

Berg, D. K., & Thongphakdee, A. (2019). In vitro culture of deer embryos. In J. R. Herrick (Ed.), Comparative embryo culture: methods and protocols. Methods in Molecular Biology, v. 2006 (pp. 191–207). Springer Nature. doi:10.1007/978-1-4939-9566-0_14

Usage metrics

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC